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A sterospecific colorimetric assay for (s,s)-Adenosylmethionine quantification based on thiopurine methyltransferase-catalyzed thiol methylation
(2002)
S-Adenosyl-L-methionine (AdoMet or SAM) that is biologically synthesized by AdoMet synthetase bears an s-configuration at the sulfur atom. The chiral sulfonium spontaneously racemizes to form a mixture of s-and R-isomers ...