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Optimizing the Enzyme/Prodrug Axis of Suicide Gene Therapy Using Protein Engineering Strategies
The unifying goal of cancer therapy is to selectively eradicate cancer cells while sparing normal tissue from damage. As many current anticancer therapies lack specificity and cause unwanted side effects there is an unmet ...
Isolation of ABA-response mutants in allohexaploid bread wheat (Triticum aestivum L.): Drawing connections to grain dormancy, preharvest sprouting, and drought tolerance
This dissertation describes the characterization of seed dormancy and drought tolerance phenotypes of abscisic acid (ABA) response mutants in allohexaploid wheat (
Development, Optimization and Validation of Targeted and Global Metabolite Profiling Strategies for Probing Plant Metabolism
Metabolomics is the attempt to comprehensibly identify and quantify all metabolites in a cell, tissue or organism, which is a tremendous challengedue to the enormous chemical diversity found in living systems, particularly ...
STABILIZING INTERACTIONS IN THE H2A-H2B MONOMERIC AND DIMERIC KINETIC INTERMEDIATES
The long-standing goal of protein folding studies is to understand how the amino acid sequence encodes the folding of a peptide from an extended conformation to the biologically active, three-dimensional structure. ...
CHARACTERIZATION OF MITOCHONDRIAL AND CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASES
As a folate-dependent enzyme, serine hydroxymethyltransferase (SHMT) catalyzes the reversible conversion of L-serine and (6S)-H4PteGlun (tetrahydrofolate, THF) to glycine and (6S)-5,10-CH2-H4PteGlun. The glycine-forming ...
Pleiotropic Effects of Myostatin are Associated with Differences in Stem/Progenitor Cell Pools
Myostatin is best known as a negative regulator of skeletal muscle growth, although it influences a number of physiological and developmental systems. Moreover, the myokine circulates, and based on limited studies with ...
Understanding the Physiological Significance of Ureide Transport Processes in Soybean (
The ureides allantoin and allantoic acid are the major long-distance forms of nitrogen (N) in nodulated tropical legumes like French bean (
Integrative approaches for understanding metabolism in plant specialized cell types
Plants contain specialized tissues/cell types that play important roles in the synthesis of many natural products. Investigation at cell-specific resolution is necessary for understanding the functional specialization of ...
Identifying Regulatory Components of Phytosterol Biosynthesis in the Genomics Era
The biosynthesis of sterol molecules is both ubiquitous and essential in the eukaryotic lineage. Despite our advanced knowledge of the structural genes and enzymes involved in phytosterol biosynthesis in Arabidopsis thaliana, ...